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Irvine Scientific serum substitute supplement sss
Serum Substitute Supplement Sss, supplied by Irvine Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/serum+substitute+supplement+(sss/serum+substitute+supplement+sss/med_rxiv__2025__03__25__25324518-146-8-12
Average 90 stars, based on 1 article reviews
serum substitute supplement sss - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

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Article Title: Effect of freezing and thawing on ejaculated sperm and subsequent pregnancy and neonatal outcomes in IVF
Article Snippet: Then, we added 1-mL human tubal fluid (HTF; Irvine Scientific, USA) with 5%(v/v) serum substitute supplement (SSS; Irvine Scientific, USA) to the pellet and let the sperm swim up at 37° for 60 min.

Article Title: Ischemia and vasculogenesis after transplantation of frozen and vitrified human ovarian tissue onto Chick Embryo Chorioallantoic Membrane (CAM).
Article Snippet: Objectives: The first days of post-ovarian transplantation are critical periods, as the ischemic injury can diminish the success rate.. In this study, the first day’s events of ovarian transplantation in two dimensions of structure and ultrastructure following slow freezing and vitrification were assessed.. Study design: Ovarian tissues (OTs) from 10 cancerous patients were frozen in two methods of slow freezing and vitrification.

Article Title: Carboxylated Poly-L-lysine Potentially Reduces Human Sperm DNA Fragmentation after Freeze-Thawing, and Its Function Is Enhanced by Low-Dose Resveratrol.
Article Snippet: The supernatant was discarded, and the sperm was subjected to centrifugation at 400× g for 10 min after the addition of a multipurpose processing medium (MHM; Irvine Scientific, Santa Ana, CA, USA) containing 10% v/v serum substitute supplement (SSS; Irvine Scientific, Santa Ana, CA, USA).

Article Title: Large-scale analysis of de novo mutations identifies risk genes for female infertility characterized by oocyte and early embryo defects.
Article Snippet: Briefly, IVF was performed in human tubal fluid (HTF; Irvine Scientific, Santa Ana, CA, USA) with 10% (v/v) serum substitute supplement (SSS; Irvine Scientific, Santa Ana, CA, USA) and ICSI was performed in separate microdroplets.

Cell Culture:

Article Title: Divergent composition and transposon-silencing activity of small RNAs in mammalian oocytes.
Article Snippet: .. Oocytes were cultured in Continuous Single Culture medium (CSC, Irvine Scientific, CA, USA) with 10% Serum Substitute Supplement (SSS, Irvine Scientific) at 37 °C with 5% CO2. ..

Article Title: Divergent composition and transposon-silencing activity of small RNAs in mammalian oocytes
Article Snippet: .. Oocytes were cultured in Continuous Single Culture medium (CSC, Irvine Scientific, CA, USA) with 10% Serum Substitute Supplement (SSS, Irvine Scientific) at 37 °C with 5% CO 2. ..

Suspension:

Article Title: Ultrasound-Guided Rete Testis Approach to Sperm Aspiration and Spermatogonial Stem Cell Transplantation in Patients with Azoospermia
Article Snippet: Digested tissue was centrifuged 200 × g for 5 minutes, the supernatant discarded, and then washed with fresh 1x PBS twice before digestion with 0.25mL Pulmozyme (Genentech; 1mg/mL) and 1mL of Trypzean (Sigma Aldrich, T3568; 2.5mg/mL) in PBS with 1.4mM EDTA per 100mg of tissue for 15 minutes at 37°C in a water bath. .. Digestion was terminated by adding 10% volume of Serum Substitute Supplement (SSS; Irvine Scientific, 99193) and the suspension was filtered through a 70μm cell strainer. .. Cells were pelleted by centrifugation at 600 × g for 15 minutes and resuspended in 15% SSS 10%DMSO (Gaylord and Chemical, Procipient®) 75% Quinn’s Advantage Blast Medium (Cooper Surgical, ART 1029) at a cell concentration of 1×10 7 cells/mL.

Modification:

Article Title: Significant decrease of maternal mitochondria carryover using optimized spindle-chromosomal complex transfer.
Article Snippet: .. Then, 16 to 18 h later, mice were euthanized by cervical dislocation, and cumulus-oocyte complexes (COCs) were recovered in modified human tubal fluid medium (mHTF, 90126, IrvineScientific, United States of America) supplemented with 10% serum substitute supplement (SSS, 99193, IrvineScientific) at 37 ̊C. .. The surrounding cumulus cells were denuded through a brief exposure to 5 μg/mL hyaluronidase (H4272, SIGMA, Germany).



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